1. According to the throughput
48-well/96-well: suitable for multi-channel pipettes and automated workstations, 96-well plates are the most commonly used specifications on the market;
384-well: mainly used in automated workstations, suitable for high-throughput experiments;
1536-well: specially designed for ultra-high-throughput experiments, suitable for large-scale screening;
2. According to whether the strips are removable or not
- Non-detachable ELISA plates: the strips are connected to the plate rack as a whole, and the price is cheap;
- Detachable ELISA plates: the strips are separated from the plate rack, and the single hole can be detached and used as needed to avoid waste.
3. The bottom structure of the ELISA plate is diverse, and the common ones are flat bottom, C bottom, round bottom and V-shaped bottom;
- Flat bottom: also called F bottom. Light will not be deflected when passing through the bottom, and it has the largest light transmission area, which is suitable for bottom reading experiment detection.
- C bottom: flat bottom arc guide angle, which has the advantages of flat bottom and round bottom, can provide good cleaning effect and have a large light transmission area.
- Round bottom: also called U bottom, provides the best cleaning effect and mixing, suitable for applications that require testing precipitates.
- Conical bottom: also called V bottom, suitable for precise sampling and storage of trace samples to obtain the best small-volume recovery suitable for sample storage.
4. According to adsorption capacity
- High adsorption ELISA plate: strong protein binding ability, suitable for large molecular proteins (>10kD), high sensitivity, but attention should be paid to non-specific reactions;
- Medium adsorption ELISA plate: suitable for large molecular proteins (>20kD), moderate binding ability, suitable for unpurified antibodies or antigens;
- Aminated ELISA plate: suitable for small molecular proteins, with positive charge on the surface, which can bind to negatively charged small molecules through ionic bonds;
5. According to color
- Transparent ELISA plate: most commonly used, suitable for light absorption detection, not suitable for luminescence detection
- White ELISA plate: high reflectivity, suitable for chemiluminescence and substrate colorimetric detection, high sensitivity;
- Black ELISA plate: strong light absorption characteristics, suitable for fluorescence detection, effectively eliminating background interference;
Post time: Mar-06-2025